نوع مقاله : مقاله پژوهشی
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران
2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران
3 گروه گیاه پزشکی، دانشگاه مراغه، مراغه، ایران
عنوان مقاله [English]
In order to identify free-living and plant-parasitic nematodes in East Azarbaijan province, more than 600 soil samples were collected from vegetation’s rhizosphere at different areas during 2016 and 2017. The nematodes were extracted using classic techniques, fixed and transferred to anhydrous glycerin. Microscopic slides were prepared and the nematodes identified based on morphological, molecular and morphometric characters. In this study, four species, Longidorus apulus, L. iranicus, L. persicus and L. proximus were identified. In addition, the D2-D3 region of 28S rRNA gene of the identified species were amplified and sequenced. The results of the phylogenetic analyses confirmed the species identity and the relationships of the sequenced species were discussed. In total, 13 sequences were obtained during present study and deposited in the GenBank (NCBI) database.